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Searching for a safe and virus-free Dairy Dash game download to play? Farm as the entertaining hustle of the Diner Dash series goes pastoral. Care for cows, goats. SINOPSIS FILM PASTORAL DIARY COW FARM BRIDE (2015): Bekerja keras di sebuah peternakan sapi dan menghabiskan hari donggil. Wanita yang menarik memberikan sebuah peternakan sapi dalam satu hari itu adalah karya panjang yang sama datang untuk membantu kekurangan.
Abstract
Help these city slickers get a grip on the family farm as the entertaining hustle of the Diner Dash® series goes pastoral. Care for cows, goats, chickens, and sheep while growing tomatoes, corn and pumpkins. You'll even enjoy the occasional glass of lemonade and fill an order or two for Flo. It's all in a day's work in Dairy Dash®!
With the abolition of milk quotas in the European Union in 2015, several member states including Ireland, Luxembourg, and Belgium have seen year on year bi-monthly milk deliveries to dairies increase by up to 35%. Milk production has also increased outside of Europe in the past number of years. Unsurprisingly, there has been a corresponding increased focus on the production of dried milk products for improved shelf life. These powders are used in a wide variety of products, including confectionery, infant formula, sports dietary supplements and supplements for health recovery. To ensure quality and safety standards in the dairy sector, strict controls are in place with respect to the acceptable quantity and species of microorganisms present in these products. A particular emphasis on spore-forming bacteria is necessary due to their inherent ability to survive extreme processing conditions. Traditional microbiological detection methods used in industry have limitations in terms of time, efficiency, accuracy, and sensitivity. The following review will explore the common spore-forming bacterial contaminants of milk powders, will review the guidelines with respect to the acceptable limits of these microorganisms and will provide an insight into recent advances in methods for detecting these microbes. The various advantages and limitations with respect to the application of these diagnostics approaches for dairy food will be provided. It is anticipated that the optimization and application of these methods in appropriate ways can ensure that the enhanced pressures associated with increased production will not result in any lessening of safety and quality standards.
Introduction
The European Union’s removal of milk quotas in April, 2015 led to a 2% increase in milk deliveries to dairies in the EU for 2015. Some countries are taking full advantage of the new limitless system in the EU, with Ireland, Luxemburg, and Belgium increasing bi-monthly milk deliveries to dairies by in excess of 20% (Eurostat, 2016). Although the production rate has slowed in some other major dairy exporters, including New Zealand and Australia, the US has seen continued increases in production (Dairy Australia, 2015; DCANZ, 2016; USDA, 2016). The surplus milk produced can be processed into a wide variety of dairy products, including yogurt, butter, cheeses, and dairy powders. Dairy powders are a popular commodity due to their long shelf life, ease of storage and versatile nature. A wide variety of dairy powders can be produced, each with individual properties. These include whole milk powder (WMP), skimmed milk powder (SMP), whey protein concentrate (WPC), whey protein isolate (WPI), milk protein concentrate (MPC), milk protein isolate (MPI), casein and caseinates (). Dairy powders can be used in fortification of other dairy products (), as well as an ingredient in a wide array of foods including soups and sauces, confectionary (Sharma et al., 2012), infant formula, sports dietary supplements and in foods for health recovery (; ). However, the increased production of dairy powders may create safety and economic risks to the dairy sector, specifically when controlling microbial loads in these products. Several key steps are involved in producing dairy powders including pasteurization, separation, evaporation, and spray drying (Figure Figure11). These thermal and mechanical processes can reduce the microbes present in the milk. However, spore forming bacteria may survive. It has been shown that the spore-forming bacterial composition of raw milk differs considerably from their associated dairy powders (), highlighting that the processing of milk into powder changes the composition of the specific spore-formers present. Post-production, powders can be stored for extended periods and in the absence of water, bacterial metabolic activity and growth is limited (), thus preventing spoilage and product defects. However, under these conditions, bacterial spores can remain dormant until more favorable conditions are encountered, when germination and outgrowth can proceed (, ).
Sample dairy powder production pipelines.
Bacterial Contaminants of Dairy Powders
Sources of Bacterial Contamination of Dairy Powders
Spore-forming bacteria can contaminate dairy powders through a variety of means. Bacteria can originate from the soil (Heyndrickx, 2011), feces, bedding, feed, or milking equipment (Gleeson et al., 2013), or can enter the raw milk via contaminated teats, milking cups and bulk tanks. Additionally, contamination can occur during transport from the farm to the processing plant (), and also within the processing facility itself from poor handling and contaminated equipment (; ). The formation of homogeneous or heterogeneous multicellular bacterial communities on the surface of processing equipment in the form of biofilms is a particular concern for the dairy processing sector and, when present, can lead to recurring problems of microbial contamination. The biofilms, which are themselves resistant to cleaning, can serve as a reservoir for bacterial spores which can slough off and contaminate dairy powders (; ).
Common Bacterial Contaminants
Common contaminants identified in dairy powders include species of the class Bacilli (Table Table11), many of which are capable of forming endospores (). Taxa other than Bacilli have also been found to contaminate powdered dairy products with species reported including Clostridium halophilum, Klebsiella oxytoca (), C. perfringens, C. septicum, C. novyi/haemolyticum, C. sporogenes (), Staphylococcus aureus (), and Cronobacter sakazakii (). Bacteria of the genus Clostridium, as well as many of the contaminants of the class Bacilli (Table Table11), including Bacillus, Anoxybacillus, Geobacillus, Lysinibacillus, Brevibacillus, and Paenibacillus, have a considerable advantage due to being capable of forming stress-resistant endospores. These genera, and their associated species, vary considerably with respect to the range of temperatures in which they can grow, and include some psychrophilic () and thermophilic (; ) species. Dairy product contaminating spore-formers can also differ by virtue of preferring anaerobic () or aerobic () conditions. Although many spore-formers are not pathogenic and are seen primarily as indicators of poor hygiene during milk collection and or processing (), some can cause disease (). Of the spore-formers identified in powders, specific representatives of Clostridium spp. and Bacillus spp. are the most worrying from a food safety point of view. Clostridium are anaerobic spore-formers, of which C. botulinum is the most notorious due to its highly potent botulinum toxin. There are many types of botulism including foodborne botulism, wound botulism, infant botulism and adult intestinal botulism. Infant botulism is the most common form (). Strains of C. botulinum isolated clinically have been identified in containers of opened milk powder from the home of patients with infant botulism (; ). Despite this, and although many species of Clostridium have been identified in dairy powders (; ), dairy powders have never been found to be responsible for a case of infant botulism (; ; ). However, it is worth noting that anaerobic spore-forming bacteria, like C. botulinum, are less common than aerobic spore-formers in dairy powders. This may be due to the high degree of aeration involved in dairy powder processing or that testing criteria for spore-formers has been optimized to identify aerobic spore-formers except in the case of phenotype based assays for specific groups of anaerobic species. The ability of certain Clostridium species to reduce sulphite to sulfide under anaerobic conditions resulting in black colonies on specific media has been widely utilized. The accuracy of these qualitative and quantitative approaches has previously been discussed (). Of the aerobic spore-formers identified, the majority have been of the genus Bacillus (Table Table11). Many species of this genus are generally regarded as safe and some are even used as probiotics (); e.g., Bactisubtil, Biovicerin and Biosubtyl containing B. cereus, Bidisubtilis containing B. subtilis, Biosporin and Primal Defense containing B. subtilis and B. licheniformis, Biosubtyl containing B. pumilus, Enterogermina containing B. clausii and Lactospore containing B. coagulans (). Other species of Bacillus have been used in the production of animal feed-stuffs; e.g., B. subtilis has been utilized for the fermentation of indigestible by-products of soya bean oil production to yield a suitable food source for monogastric animals (). B. cereus sensu lato is the most important group of species identified from a pathogenic perspective (). This group, containing up to 11 individual, highly related species (Okstad and Kolsto, 2011; ), includes species that are regarded as non-pathogenic (Okstad and Kolsto, 2011). Other species include B. thuringiensis which is used as pesticides (; ); B. cereus, a class 2 pathogen capable of food poisoning which gave this species group its name () and even a class 3 human pathogenic species, B. anthracis (). All of these are notoriously difficult to classify and differentiate from each other (; ; ; ). B. cereus is the main cause of food poisoning from within this group. B. cereus strains can contain many enterotoxins which are associated with diarrheal food poisoning including non-hemolytic enterotoxin (Nhe; ; ), hemolysin BL (Hbl; ), and cytotoxin K (CytK; ). It should be noted that the description of CytK as a viable enterotoxin has been called into question as, in isolation, the presence of the corresponding gene has not been linked to virulence in diarrheal pathogenesis (). Other molecules previously thought to be enterotoxins associated with food poisoning but which have since been reclassified include EntFM () and BcET (). Some strains of B. cereus also produce an emetic toxin, cereulide (Ces), a product of non-ribosomal peptide synthesis, which can cause emetic food poisoning (; ).
Table 1
Contaminants of the class Bacilli identified in powdered dairy products.
Bacilli contaminants | Reference |
---|---|
Bacillus lichenformis | Ronimus et al., 2003; Ruckert et al., 2004; Rueckert et al., 2005; Reginensi et al., 2011; Buehner et al., 2015; Miller et al., 2015; Sadiq et al., 2016; VanderKelen et al., 2016 |
Bacillus subtilis sensu lato | Ronimus et al., 2003; Ruckert et al., 2004; Rueckert et al., 2005; Reginensi et al., 2011; Miller et al., 2015; Sadiq et al., 2016 |
Bacillus pumilus | Ruckert et al., 2004; Reginensi et al., 2011; Buehner et al., 2015; Miller et al., 2015; Sadiq et al., 2016; VanderKelen et al., 2016 |
Bacillus circulans | Ruckert et al., 2004; Sadiq et al., 2016 |
Bacillus coagulans | Ruckert et al., 2004; Sadiq et al., 2016 |
Bacillus cereus sensu lato | Reyes et al., 2007; Buehner et al., 2015; Miller et al., 2015; Sadiq et al., 2016; Zhang et al., 2016 |
Bacillus megaterium | Reginensi et al., 2011; Buehner et al., 2015 |
Bacillus sonorensis | Buehner et al., 2015; Sadiq et al., 2016 |
Bacillus altitudinis | Buehner et al., 2015 |
Oceanobacillus spp. | Buehner et al., 2015 |
Bacillus clausii | Miller et al., 2015; Sadiq et al., 2016 |
Bacillus thermoamylovorans | Miller et al., 2015; Sadiq et al., 2016 |
Anoxybacillus spp. | Miller et al., 2015; Trmcic et al., 2015; Sadiq et al., 2016 |
Anoxybacillus flavithermus | Ronimus et al., 2003; Ruckert et al., 2004; Rueckert et al., 2005; Reginensi et al., 2011; Sadiq et al., 2016; VanderKelen et al., 2016 |
Geobacillus spp. | Miller et al., 2015; Trmcic et al., 2015 |
Geobacillus stearothermophilus | Ronimus et al., 2003; Ruckert et al., 2004; Rueckert et al., 2005; Buehner et al., 2015; Sadiq et al., 2016 |
Geobacillus thermoleovorans group | Sadiq et al., 2016; VanderKelen et al., 2016 |
Ureibacillus spp. | Miller et al., 2015 |
Urebacillus thermosphaericus | Ruckert et al., 2004 |
Aeribacillus pallidus | Miller et al., 2015; Sadiq et al., 2016 |
Lysinibacillus spp. | Miller et al., 2015 |
Lysinibacillus sphaericus | Sadiq et al., 2016 |
Paenibacillus spp. | Miller et al., 2015 |
Paenibacillus cookii | Sadiq et al., 2016 |
Paenibacillus macerans | Sadiq et al., 2016 |
Bacillus aerophilus sensu lato | Sadiq et al., 2016 |
Brevibacillus brevis | Sadiq et al., 2016 |
Brevibacillus parabrevis | Sadiq et al., 2016 |
Virgibacillus proomi | Sadiq et al., 2016 |
Bacillus shackletonii | Sadiq et al., 2016 |
Sporosarcina contaminans | Sadiq et al., 2016 |
Laceyella sacchari | Sadiq et al., 2016 |
Bacillus amyloliquefaciens | VanderKelen et al., 2016 |
Spore Formation
Endospores are formed in Bacillus and Clostridium species in response to environmental stress, by the activation of the master transcriptional regulator Spo0A () following a cascade of phosphorylation including five autokinases and two phosphorelay proteins (). Spo0A binds to DNA and influences the expression of over 500 genes (). It does so directly, for example it can control efficient replication of a single chromosome for both the mother cell and fore spore by binding to the origin of replication in the mother cell (). But it can also work indirectly, through regulation of other transcription factors (). There are over 100 genes known to be required for spore formation, with more being identified as research in the field develops (). Steps involved in spore formation include segregation of DNA, formation of a septum, engulfment and formation of a fore spore, formation of spore protein layers, cortex, membranes and spore coat and maturation of the spore before lysing the mother cell and being released. This process has previously been comprehensively reviewed elsewhere (; ). Following its formation, an endospore can remain dormant and can persist in unfavorable environmental conditions without moisture or nutrients due to the protective structure and properties of the endospore.
Spore Structure
Endospores contain several thick layers. The outer coat, or exosporium, is a thick layer only found in some species, usually those of B. cereus sensu lato (; ). The exosporium contains two layers, a basal layer surrounded by an external layer with hair like projections consisting mainly of the glycoprotein Bacillus collagen-like protein A (BclA; ; ). The exosporium, and especially BclA, contributes to hydrophobicity and aids the binding of spores to their substrates, including food preparation surfaces and stainless steel. This, along with its ability to assist spores in their avoidance of innate immune cells (), and also aids the spores’ survival, spread and pathogenicity potential in the food chain. The exosporium, if present, surrounds the spore coat. The spore coat is a complex, semipermeable, proteinaceous layer found on all endospores. It is the outermost layer of B. subtilis spores () and gives resistance to chemicals and enzymes, as well as structurally holding the spore together. It excludes large molecules, while allowing nutrients pass through and interact with germination receptors deeper in the spore structure (; ). The spore coat surrounds an outer membrane, which encapsulates the cortex. The cortex is made of specific peptidoglycan () that is assembled into rod shaped structures, located perpendicularly to the spore surface (). It confers resistance to wet heat and is essential in the dormancy of the spore as well as reducing the water content of the core (). The cortex surrounds the germ cell wall, which becomes the bacterial cell wall following germination (; ). The germ cell wall surrounds an inner membrane. This too protects the bacterial spore against chemicals, and contains the proteins required for germination back to active cells (). Proteins including transporters (some of which are associated with efflux processes and unique to the spore inner membrane), proteases (essential for sporulation and germination), DNA repair and replication enzymes (including nucleotide excision repair enzymes, spore specific lyases and endonucleases), heat shock proteins and proteins involved in control of cellular processes in response to stress (including, but not limited to UV and oxidative stress) have all been identified in the spore inner membrane (). These all contribute to the resistance and persistence of spores in unfavorable conditions. Inside the inner membrane is the core of the endospore, which is severely dehydrated and compacted. This dehydration allows immobilization of proteins, preventing their coagulation following heat denaturation (). The core also contains high levels (up to 15–25% of the spores dry weight) of dipicolinic acid (DPA), most of which is chelated by divalent ions, allowing protection of spore DNA from external stressors as well as synthesis of new DNA in response to UV radiation (, ; ). Also found in the spore core of Bacillus species is a group of small, acid-soluble spore proteins (SASP) of the α/β-type. These bind DNA in the spore core and alter its structure, thus aiding its resistance to heat, chemicals, UV radiation, and osmotic pressure (, ).
Survival of Spore-Forming Bacteria in Processing Environments
Spores can survive processing to which vegetative cells would normally succumb. Such processing-related stressed include desiccation, dry and wet heat, UV radiation, mechanical agitation, γ-radiation, chemical exposure and hydrostatic and osmotic pressure (; ). Indeed, while the temperatures and drying conditions used in the processing of milk to powders kills most vegetative bacterial cells, it also inadvertently selects for these spore-formers. Once powders are rehydrated, the spores may germinate by activation of germination receptors, either in response to nutrients called germinants () or by heat activation (). Germination independent of these receptors may also be triggered by calcium chelated dipicolinic acid (CaDPA), dodecylamine, or peptidoglycan fragments, although these mechanisms may not be applicable to the food industry (). Germination initiated by high pressure, either by activation of germination receptors or independent of them, can also occur (). Following germination, these spore-formers can proliferate in the absence of competition from other bacteria that were eradicated during processing ().
Legislation Governing Bacterial Contamination in Dairy Powders
Guidelines governing the levels and types of bacteria permitted in dairy powders are not very comprehensive, except in the case of infant formula. There are many different governing bodies that have set testing parameters; including the U.S. Food and Drug Administration (FDA), Food Standards Australia New Zealand (FSANZ), and The European Commission (EC). In Ireland, the Food Safety Authority of Ireland (FSAI) implements limits based on the Commission Regulation (EC) No 2073/2005 (European Commission, 2005). FSAI state that aerobic colony counts in dairy powders should ideally be <104 cfu/g (FSAI, 2014). However, this is not a legal obligation, and does not mean that the food is unsafe as characterization of the species isolated would need to be performed in order to determine product safety. The U.S. Department of Agriculture (USDA) implements the following microbial limits in US extra grade dairy powders using the standard plate count; dry buttermilk <20,000 cfu/g (USDA, 2001a), dry whey <30,000 cfu/g (USDA, 2000), dry whole milk <10,000 cfu/g (USDA, 2001b), dry casein (acid) <30,000 cfu/g (USDA, 1968), instant non-fat dry milk <10,000 cfu/g (USDA, 2013), non-fat dry milk (roller dried) <50,000 cfu/g (USDA, 1984), and non-fat dry milk (spray process) <10,000 cfu/g (USDA, 2001c). The US Dairy Export Council (USDEC) implements limits for US dairy powders destined for international customers with limits on aerobic spore-formers set to between <500 cfu/g and <1000 cfu/g for thermophilic and mesophilic spores, respectively, in SMP, non-fat dry milk and WMP destined for infant powder, and <500 cfu/g and <2000 cfu/g, respectively, in SMP and WMP ().
In Australia and New Zealand, state agencies enforce limits set by FSANZ. B. cereus must be <100 cfu/g in 4/5 samples, and <1,000 cfu/g in 1/5 samples in dried milk powder and powdered infant formula products with added lactic acid producing cultures, and must be absent in five samples of 1 g in powdered infant formula. The EC regulation, as amended (European Commission, 2005) sets similar legal microbiological criteria including a limit of <50 cfu/g presumptive B. cereus in 4/5 samples and <500 cfu/g in 1/5 analyzed is set in accordance to EN/ISO 7932 (Standards, 2004).
Due to the competitive market for dairy ingredients, individual purchasers often set their own microbiological limits to ensure high standards. In many cases dairy powders will not receive any further treatments before incorporation into other products. For example, powdered infant formula manufacturers often have close relationships with the dairy powder supplier to ensure high microbiological standard are met, and set strict criteria ().
Detection of Spore-Forming Bacteria
Apart from dairy powder that is due for export from the US, no legislation thoroughly covers the enumeration or identification of all spore-formers in dairy powder. This is in spite of recent research highlighting the need for accurate spore quantification and identification (). Identification and enumeration of all spore-formers present in dairy powders allows identification of potential problematic species whether from a hygiene, quality or pathogenic perspective. This information would allow manufacturers implement more comprehensive and/or directed preventative measures () resulting in continued economic and safety confidence in the sector. Understanding composition of total spore-formers within a product contributes to a clearer understanding of the source of potential quality or safety issues should they arise and allows faster implementation of control measures (; ). Indeed, efforts have continued to be made in recent years to improve the detection and identification of spore-forming bacteria present in dairy powders (; ; ).
Culture Based Methods
Spore Count Methods
Typical spore count tests involve the heating of a reconstituted powder sample to 80°C for 12 min before cooling, culturing and enumerating colonies (Frank and Yousef, 2004; ). Highly thermo-resistant spores are selected by heating to 100°C for 30 min before cooling and culturing while numbers of especially thermo-resistant spores are quantified by heating to 106°C for 30 min, cooling and culturing. Media is incubated in the presence or absence of oxygen to select for aerobic or anaerobic spore-forming species, respectively. Incubation can also be at different temperatures. Incubation at 6°C will select for psychrophilic spore-formers, incubation at 30–35°C will select for mesophilic spore-formers and incubation at 55°C will select for thermophilic spore-formers (; ). Further analysis of isolated colonies is required in order to determine the species present, and the options available for this analysis are discussed at a later stage in this review (see Culture-Based Identification of Spore-Forming Species and Post-Culture DNA-Based Classification Methods). Total bacterial counts and spore counts, although informative, are not without their limitations. Almost a century ago it was highlighted that different media will result in different bacterial counts () and that more than just quantitative data is needed with respect to contamination of dairy products, in order to determine the significance of the contamination (). The use of various heating methods is somewhat redundant in terms of identification of different species (). However, the actual abundance of these spore-forming bacteria does differ depending on the test method used (). In order to get a clear picture of the total spore-former composition present in a powder sample through culture-based approaches, a variety of incubation conditions, temperatures, agars and, possibly, heat treatments would be needed. This highlights the need for stronger/more robust test methods to determine the abundance of (spore-forming) bacteria in dairy powders.
Culture-Based Identification of Spore-Forming Species
Numerous culture-based tests have been developed in order to help identify spore-forming bacteria. These involve the use of selective media and, in some cases, additional tests to provide further information regarding the identity of the species present. Both Bacara and Mannitol Egg Yolk Polymyxin (MYP) agars have been developed for the isolation of B. cereus. The testing used for presumptive B. cereus in Europe (Standards, 2004) involves the use of MYP agar and the hemolysis test. However, MYP has been shown to be not as selective as Bacara agar for B. cereus (), potentially leading to false positives. Some Clostridium species, the sulphite reducing Clostridia (SRCs), have the ability to reduce sulphite to sulfide under anaerobic conditions. A number of sulphite containing agars have been developed for their selection (Wilson and Blair, 1924; Gibbs and Freame, 1965; ). SRCs are identified by a black color change, however, other bacteria capable of reducing sulphite and can also grow on these media, these are referred to as sulphite reducing bacteria (SRBs) (). Other tests can involve analyzing phenotypes by visualizing morphological properties and performing biochemical tests to narrow down the possible species (; ).
Limitations of Culture-Dependent Analysis
A common limitation with all of the aforementioned methods is a requirement that the bacteria first be cultured. This can result in important difficult-to-culture species being overlooked due to inappropriate culturing conditions, temperature, aeration, and/or media type. Furthermore, colony selection may favor the selection of the largest/most plentiful colonies above the smaller/less plentiful types. Although these methods allow isolation and enumeration of culturable species, accurate identification of each species present is difficult, very time-consuming, labor intensive and can be biased. The aforementioned isolation methods can be coupled with the following, more recently developed, protein- and DNA-based methods, to provide more robust identification.
Protein-Based Methods
Enzyme Immunoassays
A sandwich Enzyme-Linked ImmunoSorbent Assay (ELISA) has been developed for the detection of whole cells of B. cereus, by recognizing surface antigens specifically associated with B. cereus cells. This assay was developed by multiple location immunization of animal models with whole cell immunogen to develop hybridomas and subtractive screen was used to eliminate cross reactivity with closely related species (). The subtractive screen ensured the mAbs are highly specific against B. cereus and the assay has a lower detection limit of 0.9 × 103 cells/ml in phosphate buffered saline. This assay has been tested using food samples spiked with various pathogens without the need for culturing. It was highly effective at identifying B. cereus cells in mixed samples, without interference by the food matrix or influence by other related species. Although this ELISA for detection of surface antigens is specific for B. cereus, it is not clear if it can recognize spores as well as vegetative bacteria, or if it can distinguish between live and dead B. cereus (). Failure to detect spores could lead to a false negative result, whereas detection of free floating antigens from dead B. cereus cells could lead to false positive results. Additional culturing may be needed to detect cell numbers below the lower detection limit, and thus eliminate these concerns. Enzyme immunoassays have also been developed for the detection of B. cereus toxins (; ). Specific conditions are needed to ensure efficient protein production. Casein hydrolysate-glucose-yeast with 1% glucose is used for the production of enterotoxins in B. cereus, and 10% skim milk medium is used for cereulide production in B. cereus (). A negative result from a proteomic based assay would not imply that the bacteria is not present, rather protein synthesis might not be currently active.
Limitations of Protein Based Methods
The requirement for correct expression conditions in order to identify proteins of interest is a hugely limiting step in protein based method for species identification. This is particularly true for spore-forming bacteria, whose presence is of concern but are currently in a dormant state during sample testing. Such requirements for specific growth conditions increase the analysis time and complexity, which may not be possible for large scale analysis of many possible toxin producers in laboratory situations. Furthermore, it is expected that the proteinaceous nature of dairy samples would greatly impeded the sensitivity of any protein analysis performed without initial culturing, even if expression was occurring.
DNA-Based Methods
Post-Culture DNA-Based Classification Methods
Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)
Random amplified polymorphic DNA polymerase chain reaction uses short random primers to amplify multiple random DNA segments which, once visualized on an agarose gel, give unique patterns (). Analysis of these fingerprints allows differentiation of species and strains by comparing profiles of various known strains (, ). This method has been applied to colonies obtained from dairy powders in New Zealand to identify Geobacillus stearothermophilus, Anoxybacillus flavithermus, Bacillus licheniformis, and B. subtilis as the main contaminants of WMPs and SMPs, as well as buttermilk and goat milk powders (). It has also been applied to whole and SMPs in Uruguay, correctly identifying the presence of B. licheniformis, B. megaterium, B. pumilus, A. flavithermus, and B. subtilis (). Indeed, using this approach, G. stearothermophilus, A. flavithermus, and B. licheniformis have been identified as the dominant species in whole and SMPs from multiple countries including; Poland, Germany, Switzerland, France, Portugal, Netherlands, Great Britain, Ireland, Canada, USA, Mexico, Chile, Brazil, South Africa, Thailand, Australia, and New Zealand. B. subtilis, B. circulans, Ureibacillus thermosphaericus, B. coagulans, and B. pumilus have also being identified, albeit in lower quantities (). A common feature of the RAPD-PCR approach is the highlighting of the 3–4 most dominant species. However, species of lower abundance might be the most interesting in terms of food security and spoilage. One exceptional study described the use of RAPD-PCR, and revealed a more in depth array of species, in Chinese dairy powders (Table Table11) (). Apart from identifying previously unreported species, other details worth noting are that B. licheniformis, G. stearothermophilus, and A. flavithermus were again established as being present in high abundance while, importantly, B. cereus group species were also identified. This observation obviously has implications for food safety (). Although informative, analysis of the gel bands in RAPD PCR is very subjective allowing errors in classification and bias. Furthermore, the method requires time-consuming and laborious preparation of reference strains and there may also be variability between gels with the same samples, thus large-scale analysis would be difficult.
Sequencing housekeeping genes
Housekeeping genes are genes that are essential for the functions of the cell and viability of the organism, and thus typically contain highly conserved regions (; ). Genes that contain such highly conserved regions at either end of a more variable region are particularly useful for strain identification purposes as the conserved regions can be targeted using degenerate primers to facilitate PCR amplification and sequencing of the variable region (). Identification of genera present is facilitated by comparison with databases of corresponding variable region sequences of known origin (). Many genes have been utilized for classification of species in fluid milk in the form of molecular typing (Durak et al., 2006). Other typing methods have been described for milk powder isolates of Geobacillus spp. and B. licheniformis based on variable number tandem repeat analysis (; ). The 16S rRNA gene is ubiquitous among bacteria, and contains multiple conserved and variable regions making it extremely useful, in general, for taxonomic classification. However, 16S rRNA gene sequencing cannot differentiate between closely related species or subtypes and other housekeeping genes such as gyrB or rpoB have been utilized to do so (Durak et al., 2006; ). Recently, both the rpoB and 16S rRNA genes have been used to characterize the contaminating psychrophilic, mesophilic, and thermophilic spore populations isolated from sweet whey, WPC, non-fat dry milk and acid whey powders. At least 14 different species were identified, with B. licheniformis, Geobacillus spp., and Anoxybacillus spp. being the most abundant (). These methods have the potential to allow identification and monitoring of persistent species and subtypes throughout dairy powder processing plants (; ). Although not currently employed in sequencing dairy powder isolates, cpn60 (Durak et al., 2006; ), pycA, ccpA (), and groEL () have all been used to varying success in the sequencing of isolates from fluid milk (Durak et al., 2006), vaginal () and marine () populations and remain as potential targets for future application to study dairy powder-associated microbes.
Pyroprinting
Pyroprinting utilizes sequencing by synthesis on multiple copy polymorphic loci simultaneously. The sequence reads are digitalized and can be compared using Pearsons correlation distance matrix to identify strains (). This method has been developed and utilized for source tracking, i.e., tracing sources of microbial contamination in end products or, more specifically, of endospore-forming bacilli in raw milk through to dairy powders. Presumptive species identified in powder included G. thermoleovorans, A. flavithermus, B. licheniformis, B. pumilus, and B. amyloliquefaciens (). These results correlate well with previous studies on raw milk and powders using the Sanger sequencing approach (Durak et al., 2006; ).
Limitations
All of the above tests allow identification of the most abundant culturable species identified in dairy powders. However, they are limited by an initial requirement for culturing and, unless these methods are modified for identification of species directly from dairy powders, they are not suitable for the identification of non-culturable species or species of lower abundance which can be out competed when culturing, unless selective media is employed. Ultimately, while promising, these methods when compared to culture-independent sequencing (see Next Generation Sequencing for the Identification of Dairy Powder Contaminants) are labor intensive and time consuming.
Targeted DNA Based Approach
A more targeted approach can be taken in the food sector to detect specific pathogens or groups of interest. These assays allow detection of toxin genes, possible pathogenic groups or members of a species of interest. Most of these have been adapted to allow amplification directly from mixed DNA extracted from foodstuffs and thus avoid the limiting step of culturing. Many also allow quantification of the species/toxin gene containing group. Of particular relevance to this review is the fact that a great deal of research has been performed with respect to such assays and the B. cereus sensu lato.
PCR assays
Polymerase Chain Reaction (PCR)-based assays have been developed for the detection of B. cereus toxin genes. Taqman quantitative PCR (qPCR) assay of a single component of the hemolysin toxin gene in B. cereus has been developed (), amplifying the sequence corresponding to one component of one tripartite toxin. It has been reported that the Taqman probe is specific for B. cereus strains that contain this gene, however, not all B. cereus strains contain the hemolysin gene (). This assay reportedly does not give false positives with related species, such as other members of the B. cereus sensu lato including B. thuringiensis and B. mycoides. However, this assay could lead to false negatives. The assay may fail to detect other species that have the toxin genes, or other strains of B. cereus that do not have this particular toxin, but may be pathogenic due to the presence of other toxins. This assay also gives accurate quantification of viable B. cereus by comparison to standard curves. Multiplex endpoint PCR of toxin genes has also been performed to identify B. cereus in dairy samples. These assays included primers to amplify single components of B. cereus enterotoxin genes, i.e., those encoding Nhe, CytK, and Hbl () as well as enterotoxin FM (EntFM) and emetic toxin Ces (Forghani et al., 2015). However, the specificity of these assays was only tested using B. cereus and non-Bacillus species. Multiplex PCR of multiple components of B. cereus toxin genes has also been performed on single bacterial colonies isolated from dairy products and environments (). This approach allows detection of all components needed to produce viable enterotoxins, and thus lessening the chance of false readings compared to other assays that only identify one toxin gene component. Multiplex endpoint PCR assays have also been developed for hygiene indicator species, G. stearothermophilus and A. flavithermus isolated from dairy powders. These assays rely in the species specific conserved regions of ITS 16S-23S rRNA region and the rpoB gene (). Further validation of these assays could lead to their use on DNA isolated directly from dairy powders. Finally, droplet digital PCR (ddPCR) allows precise, absolute quantification of a target DNA sequence. The DNA is encapsulated into many water in oil emulsion droplets and a PCR performed on each (). This culture-independent method has recently been used to detect B. cereus in fluid milk and can provide absolute quantification without need for comparison to standard curves. In this instance ddPCR was implemented using primers that target the gyrB gene of B. cereus sensu lato and the assay was found to have a lower detection limit than traditional qPCR (), which is ideal for dairy powders that have low levels of contamination.
Biosensors
The assays described above also have the potential to be employed in the form of biosensors. Indeed, biosensors are already being developed for detection of a toxin gene found in B. cereus in milk and powder (). These biosensors are DNA based pencil graphite electrode (PGE) biosensors, in which a nhe toxin gene primer is immobilized on gold nanoparticles. Positive results are measured by an increase in charge resistance on the biosensor from the hybridization of the target DNA to nhe toxin sequence.
Limitations of targeted DNA assays
Although these methods do not give a complete view of the microbial composition in a dairy powder, they are useful as a test for key spoilage and pathogenic bacteria, including producers of harmful toxins. It is important to note that B. cereus sensu lato toxin genes are not specific to any one species of the group, nor is one toxin found in all B. cereus (; ; ). However, targeting toxins allows detection of all possible pathogenic species. Singleplex assays that target one component of one toxin may be prone to false negatives (), i.e., producers of other toxin types being overlooked, thus underestimating the number of pathogenic B. cereus cells in a sample. Multiplex assays targeting many toxins, are more robust and can be beneficial for the food industry as they are a good indicator of potential food pathogens. Targeting all components of a toxin system may be required to confirm if there is a true potential for toxin production. Furthermore, while the genes for toxins may be present, it is unclear from these assays whether any active proteins are functionally expressed. The alternative use of a non-toxin gene for identification of B. cereus (gyrB) does not distinguish between members of B. cereus sensu lato, nor does it identify if the species identified are capable of being pathogenic. Overall the detection of toxin and species specific genes are a good indicator of potential pathogenic and other species of interest being present. Although issues remain, future improvement and development should result in the full potential of these approaches being realized.
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Culture-Independent, Non-targeted DNA Analysis
As outlined, there are limitations associated with the aforementioned culture-dependent and targeted assays. Culture-independent DNA-based analysis should be considered when striving to obtain an overview of all (i.e., culturable and non-culturable) spore-forming species present in dairy powders. This involves a shift away from testing for and identifying only specific known spore-forming bacteria in order to eliminate the possibility of currently unknown or underappreciated microbiology-related food security threats.
Next generation sequencing for the identification of dairy powder contaminants
In the last decade, considerable advances have meant that next generation DNA sequencing platforms have surpassed traditional Sanger sequencing platforms in terms of speed and potential applications. Their initially extremely short sequencing read lengths are less of a concern as sequencing lengths of Illumina and Ion platforms have increased () and new, even longer read, platforms have been developed by PacBio and Oxford Nanopore (; ). The advantages and disadvantages of the various sequencing platforms have been previously reviewed elsewhere (). Regardless, research laboratories now have a much greater choice when determining which sequencing technology to use, though it should be noted that results generated using different methods, technologies or bioinformatics pipelines are not always consistent (). Whole genome shotgun sequencing is the process by where the whole genome of a single colony is sequenced. The DNA is extracted and sheared it into small pieces, before sequencing of these pieces and the use of computer software to assemble these sequences reads back together. This process can be applied to metagenomics, the term used to denote all of the genomic information from an entire community of different cells, for example the contaminants in dairy powders (). The application of metagenomic techniques to the analysis of dairy products presents exciting opportunities. Metagenomic sequencing eliminates the need to culture, thus reducing bias, and allows the identification of species that are difficult to, or cannot be, cultured in the laboratory. Metagenomic sequencing has been applied to single gene products, such as the aforementioned 16S rRNA gene that can differentiate between all bacteria present to the genus level, while the spo0A gene has been targeted to specifically identify spore-forming Firmicutes in mixed populations. A whole metagenome ‘shotgun,’ i.e., untargeted, approach has also been attempted and comparison of 16S amplicon sequencing, spo0A amplicon sequencing and metagenomic shotgun sequencing performed for the identification of Firmicutes in metagenomic samples (). Each method has advantages and disadvantages. Amplicon sequencing is more cost effective, high throughput and rapid but often only gives accurate classification to genus level, and may over-estimate microbial diversity in the sample (; ). In contrast, shotgun sequencing is more expensive, less samples can be analyzed at one time, but it gives the opportunity to accurately classify to species level provided there are accurate reference databases to compare sequence reads to . Shotgun sequencing also reduces the bias of amplicon sequencing that can arise due to need for an initial PCR amplification and, where relevant, variable gene copy numbers (; ). The other advantage of shotgun metagenomic approaches is that additional information regarding other genes of interest within the microbial community can be generated. Such genes include toxin genes (; ), sporulation genes (), non-ribosomal peptide synthase (NRPS) gene clusters (), antibiotic resistance genes (), and phage genes (), all of which may be interesting from a food safety point of view. The sequencing reads from this approach can be difficult to analyze as they can be biased toward genomes of higher abundance. This is a particular issue when studying samples from specific human and animal microbiomes where there is a considerable amount of DNA from host cells present (). It is important to note that, due to the high sensitivity of shotgun metagenomic sequencing, care needs to be taken to ensure the absence of contaminating cells or DNA from other environments (; ).
Regardless of the sequencing approach taken, bioinformatic expertise is needed to analyze sequencing data and compare sequence reads to databases. Databases and bioinformatics software are updated continuously and newer, more accessible programs are constantly being developed (), including more targeted programs and databases specifically for food microbes (; ).
Limitations
Both amplicon and shotgun metagenomic sequencing reveal the relative abundance of bacteria in a sample. Furthermore, the quantification of total bacterial load can be achieved by coupling these techniques with qPCR or ddPCR analysis, ().
While the benefits of next generation sequencing in determining the safety and quality of dairy powders provide cause for optimism, there are several hurdles. Culture-independent DNA analyses rely on one’s ability to extract all genomic DNA directly from the substrate for analysis. Extracting DNA from dairy powder can be difficult, especially from spore-forming bacteria. Although, many studies have endeavored to optimize methods for the extraction of DNA from spores that have been spiked into food, success has been varied (; ). Furthermore, the bacterial load is likely to be lower in dried dairy powders than other environmental samples in which this sort of analysis has been previously performed, such as the gut (), soil (), and fermented food (). Low DNA concentration can be overcome through use of whole genome amplification kits (; ). Although expensive, these provide for culture independent non-targeted analysis of all bacteria present in dairy powders even if present at low cell numbers. However, these kits are notoriously susceptible to contamination () and, ideally, ultra clean laboratory environments are needed for their use ().
Isolation of DNA solely from spore-formers. There may be instances where there is a specific desire to specifically focus on the sequencing of DNA from the spore-forming community within a powder sample. Isolation of DNA solely from spores/spore-forming bacteria is a challenge. One possible method would be to perform standard spore pasteurization at 80°C for 12 min (see Spore Count Methods) (Frank and Yousef, 2004; ) or other forms of targeted vegetative cell lysis (). However, free DNA could still be present in the samples from the lysed vegetative cells. Elimination of this signal could be performed using an intercalating dye (described below). Post-heat treatment, subsequent culture-based enrichment could be employed prior to DNA extraction (Frank and Yousef, 2004; ) but, as described with respect to the culture-based approaches, this has the potential to lead to bias.
Sequencing-based approaches can also be adapted to specifically focus on spore-formers by, for example targeting of the spo0A gene for amplicon sequencing, or through focusing specifically on this gene from within shotgun sequence data. However, yet again, the need to ensure optimal DNA extraction and the removal of DNA from dead cells is a key consideration. A less conventional way of overcoming such challenges could involve the isolation of spores from dairy powder using density gradient centrifugation ().
As noted above, free DNA from lysed vegetative cells can be present in samples following heat-treatments. Elimination of this signal could be performed using an intercalating dye. The use of intercalating dyes is especially relevant in the case of amplicon metagenomic sequencing where PCR amplification is performed (). This has been performed utilizing the dyes propidium monoazide (PMA) or ethidium monoazide bromide (EMA) to bind free DNA in the samples (; Forghani et al., 2015; ; ). Further testing and optimization would be needed to determine if its results are as promising for dairy powder samples with mixed populations. There are contradicting studies with regard to whether EMA or PMA is best for particular applications (; ). Very few studies have compared EMA and PMA in mixed populations, though EMA was reported to be favorable at penetrating heat damaged bacterial cells in fish fillets (). EMA has been known to penetrate some live bacteria (; ) whereas PMA has been seen not to penetrate all dead cells (). The concentrations of EMA used has seen a decrease in recent years (possibly to circumvent the penetration of live cells) and, so, while early studies used 100 μg/ml (; ), more recent studies used 8–10 μg/ml (; ). Alternatives, including the use of platinum () to bind extracellular DNA, appear promising as they have been reported to be more selective at differentiating live/dead E. coli and C. sakazakii than PMA in water and milk. Ultimately, optimization needs to take place to develop the system that is best suited to the low microbial load of mixed populations present in powdered dairy products. It should also be noted that these approaches are not effective when performing metagenomic shotgun sequencing, as there is no amplification step to eliminate the dye-bound DNA.
Outlook
Currently culture-independent, population-based, analysis is relatively expensive and, thus, further developments are needed to increase its relevance to the food industry. It is, however, becoming more accessible as a test method for companies to strategically analyze processing pipelines and end products, allowing development of targeted treatments and intervention strategies against persistent or troublesome microorganisms. To provide thorough and reproducible analysis of dairy powders in this fashion, it will be particularly important to arrive at a consensus regarding the standardized sample preparation, use of specific sequencing platforms and analysis methodologies to facilitate comparison across multiple investigations ().
Conclusion
Newer technologies have paved the way for an overhaul in the approaches taken to detect and enumerate of spore-forming bacteria in dairy powders. This can lead to a more accurate, high throughput system. Although the newer technologies themselves are not without their limitations, they are continuously improving. Optimization of these newer technologies could lead to their routine use, allowing development of improved targeted treatments and preventative measures in the powder processing industry.
Author Contributions
AM drafted and edited the manuscript. Revised and edited by CF, CH, and PC.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The reviewer HC and handling Editor declared their shared affiliation, and the handling Editor states that the process nevertheless met the standards of a fair and objective review.
Footnotes
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Funding. Research is funded by Department of Agriculture, Food and the Marine (DAFM) under the FIRM project ‘SACCP.’
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